小鼠实验性自身免疫性脑脊髓炎模型中CD4+ T细胞表面CD1d分子对多发性硬化疾病进展的影响

摘要

Objective To investigate the effect of CD1d molecules on the surface of CD4 +T cells on the progression of experimental autoimmune encephalomyelitis (experimental, allergic, encephalomyelitis, EAE) mouse models.Methods EAE model of C57BL/6 mice was established, Splenic cells were isolated at different stages of the progression of the disease .The proportion of CD1d+cells on the surface of activated and non activated CD4 +T cells was detected by flow cytometry .Results The proportion of CD1d+cells in the control group (normal group and complete Freund′s adjuvant (CFA) group),in the peak and recovery period of disease in the EAE group were compared .The proportion of CD1d+cells in the control group was (8.98 ±0.36)%, and the proportion of CD1d+cells in the peak and recovery period of disease in the EAE group were respectively ( 2.14 ±0.15 )% and ( 13.80 ±0.84 )%.The differences were statistically significant difference ( P ＜0.05) .Conclusion The trend of the proportion of CD4 +T cells expressing CD1d molecules during the course of EAE pathogenesis is verified , which lays a foundation for further study on the interaction between CD4 +T cells and NKT cells in the progression of EAE models .%目的 探讨CD4 +T细胞表面CD1d分子对实验性自身免疫性脑脊髓炎(EAE)小鼠模型疾病进展的影响.方法 建立C57BL/6小鼠的EAE模型,在疾病进展的不同时期分离小鼠脾细胞,用流式细胞术检测活化与非活化CD4 +T细胞表面的CD1d+细胞比例.结果 对照组(正常组和CFA组)、EAE组发病高峰期以及疾病恢复期相比较,对照组表达CD1d分子的CD4 +T细胞数量百分率为(8.98 ±0.36)%,EAE组高峰期及恢复期表达CD1d分子的CD4 +T细胞数量百分率分别为(2.14 ±0.15)%、(13.80 ±0.84)%,差异有统计学意义(P＜0.05).结论 验证了EAE发病过程中表达CD1d分子的CD4 +T细胞数量的变化趋势,为进一步研究EAE模型病程进展中CD4+T细胞和NKT细胞之间的相互作用奠定基础.