一株对硝基苯胺降解菌Microbacterium sp.PNA8的分离鉴定及其降解条件研究

摘要

A bacterial strain capable of utilizing p-nitroaniline as sole carbon source to growth was isolated from sewage sludge. This strain was identified as Microbacterium sp. PNA8 based on its morphology, physiological, biochemical properties and 16S rRNA sequence analysis. Results indicated that the optimal temperature and pH for cell growth and for p-nitroaniline degradation was 30℃ and 7.0. Strain PNA8 growth and p-nitroaniline degradation was considerably faster in the presence of yeast extract. Optimum conditions, in the culture medium added to 0.4 g/L yeast extract, 0.3 mmol/L p-nitroaniline degradation rate to 100% in 4 days.%从污泥中分离得到一株能以对硝基苯胺为唯一碳源、氮源和能源生长的细菌菌株PNA8.经过对其形态特征、生理生化特性、以及16S rRNA序列分析,该菌株初步鉴定为Microbacterium sp..进一步研究表明,菌株PNA8利用对硝基苯胺生长和降解的最适温度和pH分别是30℃和7.0.培养基中添加定量酵母膏有利于菌株的生长及其对对硝基苯胺的降解.最适条件下,在培养液中添加0.4 g/L酵母膏,4 d内0.3 mmol/L对硝基苯胺降解率可达100%.