21 cold-active strains which produced cold-active cellulase were isolated from Bohai Bay. One of the strains CNY01 was a kind of Trichoderma viride and its cellulase activity was 67.30 U/mL. Through UV and DES mutation, the strain CNY086 was bred and its cellulase activity was 92.17 U/mL. The strain CNY086 had stable ability to produce cellulase. Through orthogonal experiment, the optimal condition of the fermentation medium had been tested. The suitable medium was that straw power 1.20%, bran 0.70%, (NH_4)_2SO_4 0.50%, KH_2PO_4 0.55%, in this condition the cellulase activity came to 108.55 U/mL.%自渤海湾海泥中分离21株低温纤维素酶产生菌.其中菌株CNY01为绿色木霉(Trichoderma viride),酶活力为67.30 U/mL.以该菌株为出发菌株,经UV、DES等诱变,选育出高产突变菌株CNY086,酶活力为92.17 U/mL.该突变菌株低温纤维素酶发酵具有遗传稳定性.通过单因素和正交实验确定突变菌株CNY086低温纤维素酶发酵最适培养基:秸秆粉1.20%、麸皮0.70%、硫酸铵0.50%、磷酸二氢钾0.55%,上述条件下CNY086菌株酶活力达到108.55 U/mL.
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