一株硫酸盐还原菌的分离鉴定和系统发育分析

摘要

A sulfate-reducing bacteria strain, designated D11, was isolated from an anaerobic baffled reactor treating waste water containing sulfate. The cells of strain D11 were found to be Gram-negative, non-spore-forming, slightly curved rods, 0.6 μm～0.8 μm wide and 1.8 μm～3.3 μm long, motile by a single polar flagellum, catalase positive and oxidase negative. The pH range for growth was 6.0～8.0 (optimum pH 7.0) and the temperature range for was 25℃～37℃(optimum 30℃). Strain D11 can use glucose, fructose, acetate, lactate, alcohol and propanediol as sole carbon source for growth and did not use glycerol, butanol, succinate and malate. The G+C content of the DNA was 62.7 mol%. Phylogenetic analysis based on 16S rRNA and DSR gene sequence showed strain D11 was closely related to the type strain of Desulfovibrio vulgaris Hildenborough and Desulfovibrio vulgaris DP4 (99% sequence similarity).%从处理硫酸盐废水的厌氧折流板反应器中分离得到一株硫酸盐还原菌D11,该菌株革兰氏反应阴性,无芽孢,菌体杆状稍有弯曲,宽度在0.6 μm～0.8 μm,长度在1.8 μm～3.3 μm之间,有极生单鞭毛,能运动,接触酶阳性,氧化酶阴性.菌株生长的pH范围介于6.0～8.0之间.最适pH为7.0,生长温度范围为25℃～37℃,最适温度为30℃.能够以葡萄糖、蔗糖、乙酸、乳酸、乙醇和丙二醇为唯一碳源生长,不能利用丙三醇、丁醇、琥珀酸和苹果酸.菌株DNA的G+C含量为62.7 mol%.系统发育学分析表明,该菌株与两株普通脱硫弧菌(Desulfovibrio vulgaris Hildenborough and Desulfovibrio vulgaris DP4)发育关系接近,序列相似性均为99%.