桃根癌病菌拮抗放线菌抑菌物质的分离纯化与结构鉴定

摘要

[Objective] To isolate and identify the antimicrobial active component extracted from fermentation broth of antagonistic actinomycetes strain G-19 against peach crown gall (Agrobacterium tumefaciens).[Methods] Sephadex LH-20 chromatography,HPLC and middle-pressure chromatography were used for isolation and purification.LC-MS,NMR were applied to identify structure of the active component.[Results] Compound G-19-Ⅰ was isolated and structurally identified as 1,2-benzenedicarboxylic acid,1,2-dibutyl ester (DBP);compound G-19-Ⅱ was isolated and structurally identified as 2-(4-hydroxyphenyl)-3,4-dihydro-2H-chromene-3,4,5,7-tetrol,commonly known as leucopelargonidin.[Conclusion] The material basis of the antibacterial constituents in antagonistic actinomycetes strain G-19 was further clarified.%[目的]为明确根癌拮抗放线菌G-19的抑菌活性物质.[方法]采用SephadexLH-20柱层析、高效液相色谱及中压制备色谱等技术,对桃根癌拮抗放线菌G-19发酵液中的抑菌活性成分进行分离纯化,并通过LC-MS、NMR对其结构进行鉴定.[结果]分离得到化合物G-19-Ⅰ的结构为邻苯二甲酸二丁酯(DBP),化合物G-19-Ⅱ的结构为2-(4-羟基苯基)-3,4-二氢-2H-苯并吡喃-3,4,5,7-四醇,俗称无色天竺葵素.[结论]阐明了放线菌G-19抑菌活性成分的物质基础.