印第安纳沙门氏菌对氯霉素类药物耐药性分析

摘要

[Objective] To investigate the characteristics of chloramphenicols resistance and the prevalence of resistance genes in Salmonella Indiana from chicken.[Methods] The samples were isolated from chicken hatcheries,farms and slaughterhouses in Shandong Province.The isolates were identificated and tested the drug susceptibility,designing primers for the resistance genes of chloramphenicols in strains were detected by PCR and sequence analysis.[Results] The results showed that the isolation rate of Salmonella Indiana was 23.28％.The resistance rates of Salmonella Indiana from hatcheries,farms,slaughterhouses to chloramphenicol were 50％,83.33％,93.30％,florfenicol were 83.33％,100％,100％,thiamphenicol were 63％,65％,77％ respectively.In 80 chloramphenicols resistant strains,54 strains had detected catA1 gene,74 strains had detectedfloR gene,and the 5 strains had detected cmlA gene.[Conclusion] These results indicated that the resistance rates of Salmonella Indiana to chloramphenicols were different from hatcheries,farms and slaughterhouses.The catA1 gene and floR gene were widely existed in chloramphenicols resistant strains.%[目的]调查鸡源沙门氏菌对氯霉素类药物的耐药特征和相关耐药基因的流行情况.[方法]从山东省部分地区鸡孵化场、养殖场、屠宰场分离样品进行沙门氏菌鉴定和药物敏感性试验；设计引物,对氯霉素类药物的耐药基因进行PCR扩增和序列分析.[结果]试验分离到印第安纳沙门氏菌,分离率为23.28％.孵化场、养殖场、屠宰场印第安纳沙门氏菌对氯霉素耐药率分别为50.00％、83.33％和93.30％,对氟苯尼考耐药率为83.33％、100％和100％,对甲砜霉素耐药率为63％、65％和77％.在80株氯霉素类耐药菌株中,54株检测到catA1基因,74株检测到floR基因,5株检测到cmlA基因.[结论]不同来源印第安纳沙门氏菌对氯霉素类药物耐药率存在差异,catA1和floR基因广泛存在于耐药菌株中.