11个灵芝菌株的分子ID构建

摘要

[目的]收集11株灵芝菌种为材料,在分子水平上对其进行分类鉴定,并构建分子ID.[方法]采用ITS和SSR分子标记技术,对11株灵芝进行分子鉴定分析.[结果]通过内转录间隔区(ITS)序列测定分析表明,与GenBank上登录的灵芝(Ganoderma lucidum)菌株ITS序列相似度达到99％,在种的水平上证明实验所采用的供试菌株均属灵芝种(Ganoderma lucidum).利用SSR分子标记技术对菌株进行引物扩增,综合多态性条带,用NTSYS软件进行聚类分析,相似度在0.62水平上,1 1个灵芝菌种被分成4个类群,其中GL-2与GL-4各自聚为一类.用ID Analysis 1.0软件进行数据分析表明,用5对SSR引物可将11株灵芝供试菌种完全区分开,并构建其分子身份证.[结论]基于SSR分子标记构建灵芝菌属的分子ID是可行的.%[Objective] Eleven Ganoderma lucidum strains were collected as materials for classifying them at the molecular level and establishing the molecular ID.[Methods] Internal transcribed spacer (ITS) and simple sequence repeat (SSR) markers were used for the molecular identification of eleven Ganoderma lucidum strains.[Results] 99% similarity in ITS sequence between the tested strains and the Ganoderma lucidum registered in GenBank, meaning that the tested strains were Ganoderma lucidum species.The cluster analysis by NTSYS revealed that eleven Ganoderma lucidum strains were divided into four groups at similarity coefficients of 0.62.GL-2 and GL-4 were in two clades respectively.According to fragment size of allele variation, the agarose gel electrophoresis bands were analyzed by the software ID Analysis 1.0.Five primer pairs could be used to identify all the tested strains and accomplish the establishment of molecular ID.[Conclusion] Establishment of molecular ID in Ganoderma lucidum based on SSR were feasibly.