加压CO2对大肠杆菌细胞膜的损伤作用

摘要

[目的]细菌细胞膜的损伤可以表现在细菌细胞内物质泄漏和细菌细胞吸收染料.与巴氏杀菌(63℃C、30 min)比较,研究加压CO2对大肠杆菌细胞膜的损伤作用,目的是分析出大肠杆菌死亡与细胞膜损伤的关系.[方法]检测大肠杆菌细胞膜通透性的改变情况,大肠杆菌内蛋白质和核酸的泄漏程度,并通过透射电镜观察大肠杆菌形态的改变情况.[结果]在研究范围内,加压CO2处理使大肠杆菌细胞膜通透性发生改变;加压CO2处理时虽然发生了胞内蛋白质泄漏,但发生泄漏的时间明显滞后于99％以上菌体死亡时间,因此并不是大肠杆菌死亡的原因,只是大肠杆菌死亡后的继发现象；大肠杆菌死亡与加压CO2处理导致的胞内核酸泄漏有关；大肠杆菌死亡与加压CO2处理导致的菌体形态改变有关.[结论]加压CO2对大肠杆菌细胞膜的损伤作用与菌体死亡有直接关系.%[Objective] The damage in the cell membrane of bacteria could be reflected on the leakiness of the material in bacteria and the absorption of dye in bacteria.Compared with pasteurization (63 ℃, 30 min), the damaging effect of pressurized CO2 on the cell membrane of Escherichia coli was studied.The aim of the study was to analyze the relationship between the death of Escherichia coli and the damage of cell membrane.[Methods] The change of cell membrane permeability and the leakiness of protein and nucleic acid in Escherichia coli were detected.The change of Escherichia coli morphology was observed by TEM.[Results] The results indicated that pressurized CO2 treatment induced the change of cell membrane permeability of Escherichia coli.Pressurized CO2 treatment induced the leakiness of protein in Es-cherichia coli, but the time of leakiness was lagged behind the time of 99% Escherichia coli death, so it was not the reason for death, it was only the secondary phenomenon of death.The death of Escherichia coli could be related to the leakiness of nucleic acid induced by the pressurized CO2 treatment.The death of Escherichia coli could be related to the ultrastructure change of Escherichia coli induced by the pressurized CO2 treatment.[Conclusion] There was direct relationship between the damaging effect of pressurized CO2 on cell membrane of Escherichia coli and the death of Escherichia coli.