虎耳草炭疽病病原菌鉴定

摘要

[Objective] The study aims to isolate and identify the pathogen from Saxifraga stolonifera anthracnose.[Methods] The pathogen were isolated by tissue associated anthracnose and confirmed through Koch's postulates,then the strains were identified through morphologic characteristics and multi-locus (ACT,CAL,CHS-1,GAPDH,ITS,TUB2) phylogeny analysis.[Results] Two strains,isolated from the leaves ofS.stolonifera,were numbered as LPSU 20120244 and LPSU 20120251.Conidium of both two strains was hyaline,aseptate,straight,cylindrical.Conidium size of LPSU 20120244 was (11-25) μm×(5-9) μm,LPSU 20120251,(15-25) μm×(5-7) μm.Strain LPSU 20120244 and LPSU 20120251 was clustered with Colletotrichum karstii Y.L.Yang,Zuo Y.Liu,K.D.Hyde & L.Cai and C.jiangxiense F.Liu & L.Cai,respectively,and the bootstrap support of both two clades were 100％.[Conclusion] Based on morphologic characteristics and multi-locus phylogeny analysis,the two strains represent 2 Colletotrichum species,Colletotrichum karstii (strain LPSU 20120244) and C.jiangxiense (strain LPSU 20120251).%[目的]从虎耳草炭疽病病斑分离病原菌,并对病原菌进行鉴定.[方法]采用柯赫氏法则对从病斑组织块分离的病原菌进行验证,通过形态学结合多基因分子系统学方法对病原菌进行鉴定.[结果]从虎耳草炭疽病病斑分离到2株菌株(菌株号:LPSU 20120244、LPSU 20120251).2株菌株孢子均无色,无隔,直、圆柱状,菌株LPSU 20120244孢子(11-25) μm×(5-9) μm,菌株LPSU 20120251孢子(15-25) μm×(5-7) μm.多基因分子系统树中,2株菌株分别与喀斯特炭疽菌(Colletotrichum karstii Y.L.Yang,Zuo Y.Liu,K.D.Hyde&L.Cai)和江西炭疽菌(C.jiangxiense F.Liu&L.Cai)模式菌株聚为一支,支持率均为100％.[结论]形态学及多基因分子系统学分析表明菌株LPSU 20120244为喀斯特炭疽菌,菌株LPSU 20120251为江西炭疽菌.