Brucellosis is a zoonotic and contagious infectious disease caused by infection with Brucella species,threat to livestock and human healthy seriously.Prevent and eliminate to brucellosis is significant to public health security and development of breeding industry.However,the pathogenic mechanism and relative virulence factors are not clear due to the facultative intracellular pathogen and complex construction for Brucella.In addition,brucellosis vaccine are all smooth strains currently in China,leading interfere with natural infection,which cause the seriously difficult for purification.Most of research had not preferable result due to these problems.Proteomic is a new method which is developing gradually to study constitutes and disciplinarians of proteins,.the researches have increased to reveal the pathogenesis and immunologic mechanism of Brucella,and supplied a new way to solve above problems.Based on the author's own research in this field,this paper makes a summary on research progress of proteomic in the brucellosis research for seek the specific proteins and differential diagnosis methods.%布鲁氏菌病是由布鲁氏菌引起的重大人畜共患病之一,给我国养殖业发展和公共安全带来严重危害.有效控制和逐步消灭布鲁氏菌病对于公共卫生安全和养殖业的发展具有重要意义.然而,由于布鲁氏菌为胞内寄生菌且结构复杂,其致病机制和相关毒力因子仍不十分清楚;加之我国现有的布鲁氏菌疫苗均为光滑型疫苗,其诱导产生的抗体与自然感染在临床诊断上存在着干扰,给种群净化带来严重困难.虽然已有许多研究通过多种技术尝试解决上述问题,但进展多较为缓慢.蛋白质组学作为研究蛋白质组成和变化规律的新兴学科,随着其研究手段的逐步发展和完善,通过蛋白质组学的手段揭示布鲁氏菌的致病机理、免疫机制等的研究逐渐增多,对于解决布鲁氏菌带来的上述问题提供了崭新的思路.本文结合实验室自身研究,主要从蛋白质组学对布鲁氏菌特异性蛋白的挖掘和对鉴别诊断的意义等方面做一简要阐述.
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