自噬诱导剂海藻糖对帕金森症小鼠的治疗作用

摘要

Objective: To explore the effect of trehalose in the MPTP model of Parkinson’s disease. Methods: The number of surviving dopaminergic (DA) neurons in substantia nigra pars compacta (SNpc) and levels of dopamine and its main metabolites, 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) in striatum were evaluated. Re-sults: Trehalose rescued midbrain DA neurons from MPTP toxicity and increased the levels of dopamine, DOPAC, and HVA in striatum. Conclusion:Trehalose could be used for treatment of Parkinson’s disease.%目的：探讨自噬诱导剂海藻糖对帕金森症小鼠的治疗作用。方法：使用1-甲基-4-苯基-1，2，3，6-四氢吡啶盐酸盐（MPTP）建立小鼠帕金森症模型，通过观察小鼠中脑黑质区多巴胺（DA）神经元数量、纹状体内神经递质多巴胺及代谢产物变化，判断海藻糖治疗帕金森症的作用。结果：（1）免疫组化染色发现MPTP使小鼠黑质区DA神经元数量明显减少。海藻糖干预后可增加DA神经元数量。（2）HPLC检测发现MPTP造成小鼠纹状体中多巴胺明显减少，海藻糖组可增加神经递质及代谢物。结论：海藻糖能减少MPTP造成的黑质区DA神经元丢失，增加纹状体中多巴胺的含量，海藻糖能有效治疗帕金森症。