支架材料聚乳酸-羟基乙酸/磷酸三钙的细胞毒性检测

摘要

Objective To detecte the cytotoxicity of the PLGA/TCP (poly(lactic-co-glycolic acid)/Tricalcium phosphate) composite that based on the precedent experiments conducted in Tsinghua University. Methods Compared with the PLGA scaffold material, observated the surface and interior structure of the PLGA/TCP scaffold material by SEM (scanning electron microscope), the surface and interior of PLGA/TCP scaffold material appeared to be homogeneous porous under SEM, with fairly even porosity distribution. The pore diameter was approximately 400fim. The interpenetrative micro-pores were scattered over bigger pores' periphery with approximately circular contour and 3~5 μm in diameter. These pores were interpenetrative, the average factor of porosity was 89. 6%. And which selected rat L929 cell strain, and detected the cytotoxicity of the PLGA/TCP composite in vitro by MTT method. Results The surface and interior of PLGA/TCP scaffold material appeared to be homogeneous porous under SEM, with fairly even porosity distribution. The pore diameter was approximately 400μm. The interpenetrative micro-pores were scattered over bigger pores' periphery with approximately circular contour and 3~5 μm in diameter. These pores were interpenetrative, the average factor of porosity was 89. 6%. On rat L929 cell strain, used MTT Method to detect the cytotoxicity of the composite PLGA/ TCP in vitro, the result showed that the cytotoxicity of the PLGA/TCP composite was level I, according to the criterion, it can be considered as non cytotoxic. Conclusion This research has proved that the PLGA/TCP compound scaffold material has a more homogeneous structure, with the vesicular interior and the structure of PLGA/TCP composite is similar to natural bone trabecula, PLGA/TCP is non cytotoxicity, which satisfy the basic requirement of biological material application and provides a good experimental foundation for repairing autologous bone defect in the near future.%目的 检测新型支架材料——聚乳酸-羟基乙酸/磷酸三钙( PLGA/TCP)的细胞毒性.方法 采用扫描电子显微镜观察PLGA/TCP支架材料的表面及内部结构,并选用小鼠L929细胞株,用MTT法体外检测PLGA/TCP复合体的细胞毒性,与已被美国FDA批准应用于临床的PLGA支架材料进行比较.结果 扫描电镜下PLGA/ TCP支架材料表面及内部呈均匀多孔状,孔隙分布较均匀,孔隙直径平约400μm,在大孔的周围布满了相互贯通的微孔,外形多为近似圆形,微孔径为3～5μm,孔隙间相互贯通,平均孔L隙率为89.6％.MTT法检测显示,PLGA/TCP复合体支架材料细胞毒性为1级,可认为无细胞毒性.结论 PLGA/TCP复合支架材料具有较为均一且近似天然骨小梁的结构,无细胞毒性,符合生物材料应用的基本要求.