目的 探讨高压脉冲电流对家兔心脏功能与结构的影响.方法 健康雄性家兔60只,分别采用0、50、100、150、250、500mA的高压脉冲电流进行电击,脉宽100μs,持续电击5s.于电击前,电击后即刻及1、3、7、14、28d检测家兔心率和心电图变化,并在电击后即刻和28d取心肌组织观察心脏组织病理学改变.结果 50～500mA电击后即刻,家兔心率呈不同程度增加,心电图呈心律不齐、心肌缺血、心房纤颤、房室传导阻滞等改变,1d后逐渐恢复.病理学观察显示,50～500mA电击后即刻家兔心肌细胞见肿胀、肌原纤维分离、蒲肯野纤维肌浆凝聚等改变,28d后上述病变基本恢复.随电流强度增大,心脏的损伤效应呈加重趋势,尤其在150mA以上时明显加重,且恢复时间延长.结论 一定强度的高压脉冲电流可对心脏结构及功能造成可恢复性的损伤,且此损伤效应与电流强度有关.%Objective To explore the effect of high-voltage impulse current (HVIC) on the structure and function of rabbit heart.Methods Sixty healthy male rabbits were involved in present study and divided into 6 groups randomly ( n=10) . The rabbits were then shocked using a high-voltage pulse generator with current intensity of 0.50, 100, 150, 250 and 500mA (pulse width 1OOμs, duration 5s)respectively. The heart rate and electrocardiogram (ECG) of rabbits were detected before and 0.1, 3, 7, 14 and 28 days after the electric shock. Moreover, the myocardial tissue of rabbits was obtained immediately and 28 days after shock to observe the pathological changes.Results Immediately after electric shock of 50 to 500mA, the heart rate of rabbit increased by different degree, and the ECG showed arrhythmia, myocardial ischemia, atrial fibrillation and atrioventricular block, and the changes recovered gradually one day later. The pathological observation showed cell swelling, separation of myofibril and sarcoplasmic condensation of Purkinje fibers immediately after electric shock of 50 to 500mA, and the changes recovered 28 days after shock. The cardiac injuries aggravated with the increasing of current intensity, especially when it exceeded 150mA, and the recovery time prolonged. Conclusion High-voltage impulse current may induce recoverable injuries on heart structure and function, and the damage effect shows a correlation with the current intensity.
展开▼
