乌灵胶囊对卒中后抑郁大鼠学习记忆障碍的影响

摘要

目的 观察乌灵胶囊对卒中后抑郁大鼠学习记忆功能的影响,探讨其与认知改善及海马脑源性神经营养因子(BDNF)表达变化的关系.方法 40只成年雄性SD大鼠随机均分为对照组、模型组、艾斯西肽普兰治疗组及乌灵胶囊治疗组.每组10只.对照组不作手术及慢性应激处理,模型组用选择性右侧大脑中动脉栓塞结合连续3周慢性不可预见性温和应激方法建立卒中后抑郁大鼠模型,艾斯西肽普兰治疗组及乌灵胶囊治疗组在慢性应激处理期间分别给予艾斯西肽普兰0.2mg/(kg·d)及乌灵胶囊100mg/(kg·d),每日1次,连续21d.采用糖水偏爱测试和Morris水迷宫试验评价大鼠抑郁程度及学习、记忆功能,采用Western blotring检测大鼠海马BDNF的表达情况.结果 模型组大鼠认知功能及BDNF表达均明显低于对照组和治疗组(P<0.05).在慢性应激处理的同时,给予艾斯两肽普兰治疗后,大鼠认知功能改善,BDNF表达强度明显提高,与对照组比较无统计学差异(P>0.05).给予乌灵胶囊治疗后,大鼠学习记忆障碍改善,但异常的BDNF表达无改变.结论 乌灵胶囊能改善卒中后抑郁大鼠的学习记忆障碍,其作用机制与BDNF的表达变化无关.%Objective To evaluate the effects of Wuling capsule on learning and memory disorder induced by post-troke depression (PSD) in rats, and examine the relationship between the changes in cognitive function and the expression of brain-derived neurotrophic factor (BDNF) in hippocampus. Methods Forty male adult SD rats were randomly divided into four groups (10 each) : untreated control group, model group, escitalopram treatment group and Wuling treatment group. All rats, except those in the untreated control group,underwent a paradigm of 3-week consecutive chronic unpredictable mild stress (CMS) followed by selective right middle cerebral artery embolism to induce PSD. The sucrose preference was introduced to evaluate the level of depression and the spatial learning, and memory functions were detected using Morris water maze test. The expression of BDNF was analvzed by Western blotting. Results The cognitive function and hippocampal BDNF expression were significantly lower in model rats than in the untreated control group and the two treatment groups (P＜0.05). When escitalopram was administered once daily to the model rats at a dose of 0. 2mg/(kg ·d) for 21 days along with the procedure of CMS, the depressed behavior was improved with BDNF protein expression rose from 0.41 ± 0.07 to 0.86±0.09. Similar effects were found after treatment with Wuling capsule [1OOmg/( kg · d)]. except that the lower BDNF expression was not changed.Conclusion Wuling capsule can improve the learning and memory function in PSD rats, bat this effect is not related to the changes in BDNF expression in hippocampus.