动物源性食品中喹诺酮类药物残留的检测

摘要

建立一种采用微生物显色法对动物源性食品中抗生素残留进行初筛,之后再结合高效液相色谱-质谱(high performance liquid chromatography-mass spectrometry,HPLC-MS)法对阳性样品进行复测的分析检验方法.利用大肠杆菌(E.coli)作为指示菌,制备96微孔板,选取pH 7.4磷酸钠-乙腈缓冲液进行提取,以反应液150μL、菌液50μL(初始A600 nm0.4左右)、样品提取液100μL作为检测体系,可同时检测15种喹诺酮类药物.结果表明:所建立的微生物显色法操作简便、快捷、成本低,结果准确且易判断,对动物源性食品中喹诺酮类药物检出限为40~200μg/kg,符合国内外对抗生素残留限量的要求.对50份动物源性样品进行检测,其结果与HPLC-MS复测结果一致,说明该微生物法稳定性良好,可用于动物源性食品中喹诺酮类药物残留的初筛检测.%A new microbial chromogenic assay was developed to analyze antibiotic residues in animal-derived food and after that, the positive samples were identified by a high-performance liquid chromatography-tandem mass spectrometric (HPLC-MS) method. E.coli was used as the indicator strain to produce a 96-well microplate for detecting 15 kinds of quinolones. Muscle samples were extracted by pH 7.4 phosphate-acetonitrile buffer. This system of microbial chromogenic assay consisted of 150 μL the reaction solution, 50 μL of bacterial suspension (the initial A600 nm value was around 0.4), and 100 μL of the extract solution. The assay proved to be simple and cheap and the results were accurate and easy to interpret. The detection limits for 15 kinds of quinolones drugs were 40-200 μg/kg, meeting the domestic and international requirements for the determination of antibiotic residue limits. Consistent results were obtained for 50 samples of animal-derived food by microbial chromogenic assay and HPLC-MS, indicating that the assay could be used to detect antibiotic residues in animal-derived food with good stability.