目的 探讨梯度板法在无偿献血者ABO血型检测中的应用.方法 选取已知血型的血液标本,通过正交试验、优化梯度板法检测ABO血型正、反定型实验参数,并常规应用于无偿献血者血液标本的检测.结果 检测无偿献血者血液标本24922例,ABO血型1次判读符合率99.6％.发现流动采血车采血前初定ABO血型错误5例,A2型2例,B3型1例,不规则抗体1例.结论 梯度板法1次判读符合率高,对亚型及不规则抗体检出能力强.实现了血型实验的标准化、自动化、实验结果的数据化及网上自动发布,满足了血站简便、快速、准确的检测大批量无偿献血者血液标本的需求,值得推广应用.%Objective To investigate the application value of trapezoid micro-plate method in determining ABO blood-group in blood donors. Methods The known blood-group samples were collected. The samples and their forward and reverse test parameters of ABO blood-group were detected by orthogonal design and trapezoid micro-plate method. These test methods were routinely used for blood sample testing among blood donors. Results A total of 24 922 blood donor samples were determined by trapezoid micro-plate method, which showed that the correct interpretation at one time was 99.6%. However, 5 cases of ABO blood-group detected in moving vehicle for donation were wrong, and 2 cases of A2 group, 1 case of B3 group and 1 case of irregular antibody were also found. Conclusions The correct interpretation at one time of trapezoid micro-plate method to detect ABO blood-group is high, and the ability of trapezoid micro-plate method to detect ABO subgroup and irregular antibody is great. Trapezoid micro-plate method brings about the standardization, automation of ABO blood-group test and digitization of test results, and the test results are issued automatically on net. It is simple, rapid and accurate for trapezoid micro-plate method to detect a large number of blood donor samples, and it is worth that trapezoid micro-plate method is used to the determination of ABO blood-group extensively.
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