类风湿性关节炎患者外周血CD3+CD(16、56)+NK T细胞百分率的变化及意义

摘要

目的 探讨CD3+ CD( 16、56)+自然杀伤(NK)T细胞在类风湿性关节炎(RA)患者外周血中的变化及意义.方法 采用流式细胞术检测RA患者(病情活动组和病情稳定组)及健康人(对照组)外周血淋巴细胞中CD3+ CD( 16、56)+细胞的比例及CD3+ CD( 16、56)+淋巴细胞CD69的表达百分率.结果 RA病情活动组外周血CD3+ CD( 16、56)+NKT细胞的比例及NK T细胞CD69的表达百分率明显低于RA病情稳定组及健康对照组(P均＜0.01)；RA病情稳定组NK T细胞的比例及NK T细胞CD69的表达百分率与健康对照组差异无统计学意义(P均＞0.05).结论 RA患者外周血中CD3+ CD(16、56)+ NK T细胞比例减少及活性受抑制可能参与了RA的发病过程,且可能与RA的病情活动性相关.%Objective To investigate the change and significance of CD3 + CD(16, 56)+ natural killer(NK)T cells in peripheral blood of patients with rheumatoid arthritis(RA). Methods The percentage of CD3 + CD(16, 56)+ T cells and the expression of activation marker CD69 on CD3 + CD (16, 56)+ T cells in peripheral blood lymphocyte from active and inactive RA patients and healthy subjects as controls were determined by flow cytometry. Results Compared with healthy controls and inactive RA patients, the active RA patients had a lower percentage of CD3 + CD (16, 56)+ NK T cells and down-regulated expression of CD69 on CD3 + CD(16, 56)+ NK T cells (P<0.01). The percentage of NK T cells and CD69 expression on CD3 + CD(16, 56)+ T cells had no statistical significance between healthy controls and inactive RA patients (P > 0.05 ). Conclusions Low percentage of CD3 + CD (16, 56 )+ NK T cells with down-regulated expression of CD69 in peripheral blood may play a potential role in the pathogenesy of RA and be relevant to the activity of RA.