血清顶体蛋白酶抗体与精子蛋白17抗体对不孕不育症的临床价值探讨

摘要

目的 探讨血清顶体蛋白酶抗体(AcrAb)与精子蛋白17抗体(Sp17Ab)对不孕不育的影响及意义.方法 采用酶联免疫吸附试验(ELISA)检测60例不明原因不孕不育症患者及48名正常生育者(对照组)血清AcrAb、SP17Ab,采用t检验和直线相关分析进行统计.结果 不明原因不孕不育症组男性及女性血清AcrAb分别为(22.39 ±3.15)、(21.02±3.56) U/L,均明显高于对照组男、女性[(11.29 ±2.15)、(10.23±2.12) U/L,P＜0.01]；不明原因不孕不育症组男性及女性血清Sp17Ab分别为(10.47 ±2.10)、(9.63±2.04) U/L,均高于对照组男、女性[(3.63±1.05)、(3.58±1.03) U/L,P＜0.01]；男性及女性血清AcrAb和Sp17Ab之间均无相关性[相关系数(r)分别为0.17、0.20,P均＞0.05].结论 不明原因不孕不育症患者血清AcrAb及Sp17Ab含量明显高于正常人,且相互独立,可单独或联合检测作为不孕不育症的辅助诊断指标.%Objective To investigate the influence and significance of serum anti-acrosin antibodies (AcrAb) and anti-sperm protein 17 antibodies (Spl7Ab) in infertility. Methods The levels of serum AcrAb and Spl7Ab were detected by enzyme-linked immunosorbent assay ( ELISA) in 60 patients with unexplained infertility and 48 healthy subjects (control group). The results were analyzed by t test and straight line related analysis. Results The levels of serum AcrAb in males and females were (22. 39 ±3. 15) and (21. 02 ±3. 56)U/L in the unexplained infertility group, which were significantly higher than those in the control group [ (11.29 ±2. 15) and (10.23 ±2. 12)U/L,P <0. 01 ]. The levels of serum Spl7Ab in males and females were (10. 47 ±2. 10) and (9. 63 ± 2. 04) U/L in the unexplained infertility group, which were significantly higher than those in the control group [ ( 3. 63 ± 1.05 ) and (3.58±1.03)U/L, P <0. 01 ]. There was no correlation of serum AcrAb with Spl7Ab in males and females [ coefficient of correlation (r) = 0. 17 and - 0.20, P > 0.05 ]. Conclusions The levels of serum AcrAb and Spl7Ab in patients with infertility are obviously higher than those in the healthy subjects, and they are independent and can be alone or jointly detected as the indicators of infertility auxiliary diagnosis.