Objective To evaluate the clinical application significance of Sysmex UF-1000i urine formed element analyzer ( UF-1000i analyzer) for screening funguria. Methods The precision, linear range and carryover rate of Candida albwans were detected by UF-1000i analyzer. A total of 200 urine samples which UF-1000i analyzer showed like yeast cell( YLC) positive and 400 YLC negative urine samples were detected by manual microscopy. Results For UF-1000i analyzer detecting Candida albicans, the precision was 3. 83%- 4. 71% , the linear range was 10. 0-5 873. 1 cells/ΜL, the correlation coefficient was 0.999, and the carryover rate was 0. 12%. Manual microscopy was as the gold standard, and the result of receiver operating characteristic ( ROC) curve showed the best cut-off value for UF-1000i analyzer detecting YLC was 18.05 cells/ΜL. The sensitivity, specificity, accuracy, positive predictive value and negative predictive value of UF-1000i analyzer in the diagnosis of funguria were 97. 0% ,85.6% ,87. 5% ,57.4% and 99. 3% , respectively. Conclusions UF-1000i analyzer can provide quantitative results of fungal spores. It aids to diagnose urinary tract fungal infection.%目的 评价Sysmex UF-1000i尿有形成分分析仪(简称UF-1000i)在真菌尿筛查中的临床应用价值.方法 分别测定UF-1000i检测白念珠菌的精密度、线性范围和携带污染率.选择UF-1000i提示类酵母细胞(YLC)阳性样本200例,阴性样本400例,同时做显微镜检查.结果 UF-1000i检测白念珠菌的精密度为3.83％～4.71％,线性范围为10.0～5 873.1个/μL,线性相关系数(r)为0.999,携带污染率为0.12％.以显微镜检查为金标准,受试者工作特征(ROC)曲线结果显示UF-1000i检测真菌YLC的最佳临界值为18.05个/μL,其检测真菌的敏感性、特异性、准确度、阳性预测值和阴性预测值分别为97.0％、85.6％、87.5％、57.4％和99.3％.结论 UF-1000i可快速提供真菌孢子定量检测结果,辅助诊断尿路真菌感染.
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