TALENs技术在基因功能研究中的应用

摘要

Gene knockout is a broadly applicable approach for efficiently editing any gene of cells and organisms. The transcription activator-like effector nuclease (TALEN)technique is a newly-developed method,and it can target essentially any sequence by comprising a nonspecific DNA-cleaving nuclease fused to a DNA-binding domain,which can be engineered easily.TALEN technique with the capability to quickly and efficiently alter genes have profound impacts on biological research and get potential therapeutic strategies for genetic diseases.%基因敲除技术广泛应用于研究各类细胞和生物的基因功能。转录激活子样效应因子核酸酶技术（TALENs）是近年发展起来的一种新型基因敲除方法，其利用TALEs蛋白核酸结合域氨基酸序列与其靶位点核酸序列之间存在着恒定对应关系，从而组装出能特异性结合任意DNA序列的模块化蛋白。TALENs技术以其快速、高效的优势对生物基因研究领域产生深远影响，为遗传疾病的治疗提供了新的策略。