良性甲状腺疾病患者血清 IgE、FcεRIα及其抗体水平分析

摘要

Objective To analyze the expressions of immunoglobulin E (IgE), FcεRIαand their antibodies in serum from patients with benign thyroid diseases and investigate their relationships with benign thyroid diseases. Methods A total of 41 Graves′disease (GD) patients, 36 Hashimoto′s thyroiditis (HT) patients, 36 thyroid nodule (TN) patients and 60 healthy subjects were enrolled.The expressions of FcεRIα, anti-IgE antibody and anti-FcεRI antibody were determined by competitive enzyme-linked immunosorbent assay ( ELISA), and the level of IgE was determined by rate nephelometry.Their differences in 4 groups and their relationships with benign thyroid diseases were analyzed.Results The levels of IgE in GD, HT and TN groups had no statistical significance compared with that in healthy control group (P >0.05).The serum levels of FcεRIαand anti-IgE antibody in GD, HT and TN groups were higher than that in healthy control group (P <0.05).The level of anti-FcεRI antibody in GD group was higher than that in healthy control group.The relationships of the serum levels of IgE, FcεRIα, anti-IgE antibody and anti-FcεRI antibody with thyroid hormone autoantibody (TRAb) were not observed in HT or TN group, but there was a negative correlation of serum FcεRIαwith TRAb (rs =-0.350, P <0.05), and there was a positive positive correlation of anti-IgE and anti-FcεRI antibodies with TRAb in GD group (rs =0.546 and 0.520, P <0.05).Conclusions There are high expressions of serum IgE, FcεRIαand their antibodies in patients with benign thyroid diseases and a significant correlation of them with TRAb in GD group.The results suggest that these antibodies may participate in the occurrence and progress of benign thyroid diseases.%目的：分析免疫球蛋白（IgE）、FcεRIα及其抗体水平在良性甲状腺疾病患者血清中的表达情况，探讨其与良性甲状腺疾病的相关性。方法收集41例 Graves′病（GD）、36例桥本甲状腺炎（HT）和36例甲状腺结节（TN）患者以及60名健康体检者（正常对照组）的临床资料及血清，采用竞争酶联免疫吸附试验（ELISA）检测血清 FcεRIα、抗 IgE 抗体和抗 FcεRI 抗体水平，采用速率散射比浊法检测血清 IgE 水平，分析4组间的表达差异并评估其与良性甲状腺疾病的相关性。结果 GD 组、HT 组及 TN 组 IgE 水平与正常对照组比较差异均无统计学意义（P ＞0．05），而 FcεRIα水平和抗 IgE 抗体水平均明显高于正常对照组（P ＜0．05）；GD 组抗 FcεRI 抗体水平明显高于正常对照组。 HT 组及 TN 组血清 IgE、FcεRIα、抗 IgE 抗体及抗 FcεRI 抗体与甲状腺激素抗体（TRAb）均无相关性。 GD 组血清 FcεRIα与 TRAb 呈明显负相关（rs ＝－0．350，P ＜0．05），抗 IgE 抗体和抗 FcεRI 抗体与TRAb 均呈明显正相关（rs 值分别为0．546、0．520，P 均＜0．05）。结论良性甲状腺疾病患者血清中高表达 IgE、FcεRIα及其抗体，并且在 GD 中与甲状腺抗体有明显相关性，提示此抗体系统可能参与了良性甲状腺疾病的发生、发展。