BD Affirm VPⅢ芯片检测技术在阴道分泌物常规检测中的应用

摘要

目的 探讨BD Affirm VPⅢ芯片检测技术在阴道分泌物常规检测中的应用价值.方法 对182例妇女阴道分泌物分别进行Nugent评分、滴虫培养、真菌培养和BD Affirm VPⅢ芯片检测,以Nugent评分、滴虫培养、真菌培养为金标准,评价BD Affirm VPⅢ芯片检测技术的应用价值,并用SPSS 19.0软件进行统计学分析.结果 与Nugent评分结果相比,BD Affirm VPⅢ芯片检测技术检测细菌性阴道病(BV)的敏感性和特异性分别为95.1%和83.8%,K值为0.80;以念珠菌培养为金标准,BD Affirm VPⅢ芯片检测技术检测念珠菌的敏感性和特异性分别为75.8%和99.0%,K值为0.85;以滴虫培养为金标准,BD Affirm VPⅢ芯片检测技术检测滴虫的敏感性和特异性分别为92.9%和100.0%,K值为0.96.结论 BD Affirm VPⅢ芯片检测技术操作简便,可同时进行3种阴道炎病原微生物的检测,且具有较高的敏感性和特异性.克服了传统检测方法的局限性,提高了检测率和准确性,具有一定的临床应用价值.%Objective To study the role of BD Affirm VPⅢ chip technology in determining vaginal secretions. Methods A total of 182 samples of vaginal secretions were determined by Nugent,trichomonad culturing,fungal culturing and BD Affirm VPⅢ,and the results of Nugent,trichomonad culturing,fungal culturing were used as gold standard. The results were analyzed statistically by SPSS 19. 0 software. Results Compared with Nugent,for bacterial vaginitis(BV),the sensitivity of BD Affirm VPⅢ was 95.1%,the specificity was 83.8%,and Kappa value was 0.80. When the results of fungal culturing were used as gold standard,for determining Candida,the sensitivity of BD Affirm VPⅢ was 75.8%,the specificity was 99.0%,andKappa value was 0.85. When the results of trichomonad culturing were used as gold standard,for determining trichomonad,the sensitivity of BD Affirm VPⅢ was 92.9%,the specificity was 100.0%,and Kappa value was 0.96.Conclusions BD Affirm VPⅢ chip technology is convenient and can be simultaneously determine 3 kinds of pathogenic microorganisms for vaginitis,and it has high sensitivity and specificity. It overcomes the limitation of traditional determination methods with clinical application significance.