碳青霉烯类抗菌药物灭活试验筛查产碳青霉烯酶肠杆菌科细菌的效能评价

摘要

目的 评估碳青霉烯类抗菌药物灭活试验(CIM)快速筛查产碳青霉烯酶肠杆菌科细菌的应用价值.方法 以碳青霉烯酶基因聚合酶链反应(PCR)及测序结果为金标准,用CIM在154株肠杆菌科细菌(分离于临床样本)中筛查产碳青霉烯酶的细菌,并与改良Hodge试验结果进行比较.结果 CIM的符合率、特异性和敏感性分别为99.4%、96.6%和100.0%,改良Hodge试验的符合率、特异性和敏感性分别为98.7%、93.1%和100.0%.结论 与改良Hodge试验相比,CIM具有符合率和特异性高、结果易于观察等优势,适合在各级医院的微生物实验室中推广使用.%Objective To evaluate the efficiency of carbapenem inactivation method(CIM)for screening carbapenemase-producing Enterobacteriaceae. Methods The results of polymerase chain reaction(PCR)and DNA sequencing for determining carbapenemase genes were used as gold standard. A total of 154 clinical isolates of Enterobacteriaceae were determined by CIM,and the results were compared with those of modified Hodge test. Results The consistency rate,specificity and sensitivity of CIM were 99.4%,96.6% and 100.0%,respectively. The consistency rate,specificity and sensitivity of modified Hodge test were 98.7%,93.1% and 100.0%,respectively. Conclusions In comparison to modified Hodge test,CIM shows high consistency rate and specificity. The observation of CIM results is easier than that of modified Hodge test. Therefore,CIM is suitable for using in all level microbiological laboratories.