杜仲叶中绿原酸的提取方法与精制工艺研究

摘要

对杜仲（Eucommia ulmoides）叶片中绿原酸的提取与精制工艺进行研究，比较了酸水提取、40%乙醇提取以及纤维素酶提取对绿原酸提取效果的影响，结果表明，酶提取更有利于后续工艺对绿原酸的精制。通过静态吸附试验，比较HPD-100A、AB-8、HPD-400A、DM-130、HPD-600、HPD-826六种大孔吸附树脂对绿原酸的吸附及脱附性能，筛选出性能较好的HPD-826树脂进行动态试验，结果表明，该树脂在室温下对杜仲叶中绿原酸的动态吸附-脱附最佳工艺参数为：上柱液pH值3.0，上柱流速2 BV/h，上柱体积7 BV；脱附剂为60%乙醇，洗脱流速1BV/h，洗脱剂用量为2 BV。用优化后的工艺进行试验，所得绿原酸粗产品的纯度可达49.85%，且工艺具有良好的稳定性。%Experiments were conducted on extracting of chlorogenic acid from leaves ofEucommia ulmoides by HCL solution, 40% ethanol and cellulose. The results showed that extracted solution by cellulose had better effect for the following purification. Comparison tests were implemented on adsorption and desorption of extracted solution of chlorogenic acid by HPD-100A, AB-8, HPD-400A, DM-130, HPD-600, HPD-826. HPD-826 had better properties and was selected for dynamic experiment. The result demonstrated that the optimal technology was as follows: 3.0 of sample pH, 2 BV/h of flow rate, 7 BV of bed volume, 60% alcohol as desorption agent, 1 BV/h of flow rate and 2 BV of desorption agent. Under the conditions above-mentioned, the purity of chlorogenic acid reached 49.85% , and the process stability was proved to be satisfactory.