Vacuum annealing was used to prepare the Ti-O powder at different annealing temperatures. The structure and surface properties were characterized by X-ray diffraction,Zeta potential test and surface area measurement respectively. Bradford method was applied to determine the adsorption and desorption of fibrinogen on the powder,and Fourier transform infrared spectroscopy was used to analyze the change of secondary structure of adsorbed fibrinogen on the samples. Then flow cytometry was used to measure the degree of platelets activation. The results show that after 800 ℃annealing process,the crystal structure of Ti-O powder transformed from anatase to rutile with 10 times less total surface area and more negative charges,and the amount of fibrinogen adsorption was decreased to 67μg and retained its normal conformation. Less platelet was activated by the annealed Ti-O powder,which proves that it has an excellent blood-compatibility.%利用真空高温退火技术制备不同退火温度的Ti-O粉末、X线衍射仪、Zeta电位和比表面积等表征材料,采用Bradford法测纤维蛋白原在其表面的吸附解吸附行为,运用红外线光谱分析纤维蛋白原在其表面吸附后二级结构的变化,用流式细胞仪研究其对血小板的激活程度。研究表明,800℃高温退火使得Ti-O粉末由锐钛矿转化为金红石,比表面积减少10倍,携带有更多的负电荷;吸附血浆纤维蛋白原的数量减少到67μg,且维持原有的二级结构,并存在较大的解吸附;不易引起血小板的激活,具有更好的血液相容性。
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