振动训练对大鼠跟腱生物力学性能影响的实验研究

摘要

The purpose of this study was to investigate different frequencies of vibration training effect on biomechanical properties of rat Achilles tendon,and to discuss functional adaptation of rat Achil-les tendon. 60 male rats 3-month-old were randomly divided into quiet control group,low,mid-dle and high frequency training group,with 15 for each group. Rats were trained twice a day with longitudinal vibration. Every time,the training time is 15 min,two training intermittent 5 min. The vibration frequency is 25Hz respectively,35Hz,45Hz,amplitude are 3 mm,5 days a week,a total of 8 weeks. After 8 weeks,the rat′s left Achilles tendon was cut out. The biomechanical parameters of rat Achilles tendon were measured by AGIS-MS material testing machine of the SHIMADZU Cor-poration. The results showed that:compared with quiet control group,in 25Hz group,the strain de-creased significantly( P <0. 05 ),the rest of the parameters increased very significantly( P <0. 01). In 35Hz group,biomechanical parameters have no significant difference(P>0. 05). In 45Hz group,the strain is no significant difference(P>0. 05),elastic modulus increased very significant-ly( P<0 . 01 ),the rest of the parameters increased significantly( P<0 . 05 ). High frequency group compared with low frequency group,the maximum load and elastic modulus have very significant difference( P<0 . 01 ),the maximum stress has significant difference( P<0 . 05 ),the rest of the parameters are not significant difference( P>0 . 05 ). It concludes that 25 Hz frequency of vibration training can signally improve the biomechanical parameters of rat Achilles’tendon,and cause func-tional adaptation.%目的：探讨不同频率的振动训练对大鼠跟腱生物力学性能的影响和功能适应性。方法：60只3月龄健康雄性SD大鼠随机分成对照组、低频组、中频组和高频组，每组15只。训练组的大鼠每天接受纵向振动训练2次，每次训练时间为15 min，两次训练间歇5 min，振动频率分别为25 Hz、35 Hz、45 Hz，振幅均为3 mm，每周训练5天，共8周。8周后，取大鼠的左下肢跟腱，使用日本岛津公司的AGIS-MS型电子万能试验机测试其生物力学性能参数。结果：与对照组相比，低频组的应变有显著的减小（P<0.05），其余参数均有非常显著的增加（P<0.01）；中频组的各参数均无显著性差异（P>0.05）；高频组的应变无显著性差异（P>0.05），弹性模量有非常显著的增加（P<0.01），其余参数有显著的增加（P<0.05）。高频组与低频组相比，最大载荷和弹性模量有非常显著的差异（ P <0.01），最大应力有显著的差异（ P <0.05），其余参数无显著的差异（ P>0.05）。结论：25 Hz的振动训练能显著地提高大鼠跟腱的生物力学性能，产生功能适应性变化。