6周递增负荷运动对大鼠小肠集合淋巴结细胞线粒体凋亡途径的影响

摘要

研究6周递增负荷运动对小肠集合淋巴结(PP结)线粒体膜电位(ΔΨm)、BaxmRNA、Bcl-2mRNA和细胞色素C(Cyt C)的影响,探讨PP结淋巴细胞线粒体凋亡途径指标的变化。将64只8周龄雄性SD大鼠随机分为运动组和对照组,运动组进行6周的递增负荷跑台训练,分别于第0、2、4、6周末,利用FQ-RT-PCR技术测定Bax和Bcl-2mRNA的表达水平；利用JC-1染色流式细胞术检测PP结淋巴细胞的平均J-aggregate( FL2)和J-monomer( FL1)的含量,并计算ΔΨm( FL2/FL1)；用ELISA法测定Cyt C的浓度。结果显示：(1)6周递增负荷运动过程中,在第2、4、6周,PP结淋巴细胞的ΔΨm显著低于0周,第4周的ΔΨm最低；(2)与0周相比,第4、6周PP结BaxmRNA表达明显增加,Bcl-2mRNA表达降低,Bcl-2/Bax值明显降低；(3)与0周相比,第2、4、6周PP结Cyt C浓度明显增加。结果表明递增负荷运动可能是通过影响线粒体途径从而影响PP结淋巴细胞凋亡的。%The authors studied the effects of 6-week load gradually increased exercising on the membrane potential (ΔΨm), BaxmRNA, Bcl-2mRNA and Cytochrome C (Cyt C) of mitochondria in Peyer’s patches, and probed into the changing of such an index as mitochondrial apoptosis pathway in Peyer’s patches. The authors divided 64 8-week old male SD rats randomly into an exercising group and a control group, let the rats in the exercising group do a load gradually in-creased exercise on a treadmill for 6 weeks, measured the levels of Bax and Bcl-2mRNA expression by utilizing the FQ-RT-PCR technology and the average contents of J-aggregate ( FL2 ) and J-monomer ( FL1) of lymphocyte in Peyer’s patches by utilizing JC-1 staining flow cytometry at the end of weeks 0, 2 4 and 6 respectively, calculated ΔΨm ( FL2/FL1), and measured the concentration of Cyt C by apply the ELISA method. The authors revealed the following findings:1) during 6-week load gradually increased exercising, the ΔΨms of lymphocytes in Peyer’s patches calculated in weeks 2, 4 and 6 were significantly lower than that calculated in week 0, the ΔΨm calculated in week 4 was the lowest;2) as compared with those measured in week 0, measured in weeks 4 and 6, the levels of BaxmRNA expression in Peyer’s patches increased significantly, the levels of Bcl-2mRNA expression decreased, and the ratio of Bcl-2 to Bax decreased significantly;3) as compared with those measured in week 0, the concentrations of Cyt C in Peyer’s patches measured in weeks 2, 4 and 6 increased significantly. The said findings indicated that load gradually increased exercising might affect the apoptosis of lymphocytes in Peyer’s patches by affecting the mitochondrial pathway.