石楠不同部位提取物抗氧化活性

摘要

为考察石楠(Photinia serrulata Lindl.)抗氧化活性与总原花青素、总酚和总黄酮质量分数之间的关系以及提取溶剂对其抗氧化活性的影响,采用不同溶剂对石楠不同部位进行了超声提取;测定了总原花青素、总酚和总黄酮质量分数;以DPPH自由基清除活性、ABTS自由基清除活性、铁离子还原能力和铜离子还原能力综合评价了其抗氧化能力.结果表明:石楠不同部位抗氧化能力差异显著,枝-韧皮部和果序-轴抗氧化活性最强,这与高质量分数的总原花青素、总酚和总黄酮有关;不同溶剂的提取效果具有显著性差异,枝-韧皮部的总原花青素质量分数为(10.77±0.08)％～(29.31±0.69)％;总黄酮质量分数为(2.60±0.02)％～(7.02±0.01)％;总酚质量分数为(3.15±0.02)％ ～ (6.42±0.07)％,其中85％甲醇提取总原花青素效果最好,70％丙酮提取总酚和总黄酮效果最好.相关性分析表明,石楠不同部位总原花青素、总酚和总黄酮质量分数与抗氧化活性之间呈现极显著正相关(P＜0.01),说明石楠总原花青素、总酚和总黄酮可能作为石楠抗氧化成分协同发挥抗氧化活性.%To investigate the relationship between antioxidant activity with total proanthocyanidins (TOPC),total polyphenols (TP) and total flavonoids (TF) of Photinia serrulata Lindl.,and inspect the influence of solvent against antioxidant activity,different parts were extracted by ultrasonic method with different solvent systems.The contents of TOPC,TF and TP were determined,the antioxidant activity was evaluated with the scavenging rates of DPPH,ABTS free radicals and FRAP,CUPRAC as indexes.The antioxidant activity has a significant difference between different parts of P.serrulata,whereas branch phloem and infructescence stalk were more potent than the other parts due to their higher contents of TOPC,TP,and TF.There were significant differences among different solvents,which showed that TOPC was ranged from (10.77±0.08)％ to (29.31±0.69)％,TF was ranged from (2.60±0.02)％ to (7.02±0.01)％,TP was ranged from (3.15±0.02) ％ to (6.42±0.07)％ for branch phloem,85％ methanol was best for TOPC,and 70％ acetone was best for TP and TF.A significant linear correlation (P＜0.01) between TOPC,TF,TP and antioxidant activity indicated that TOPC,TP and TF were jointly responsible for their antioxidant activity.