越橘品种'Koralle'茎尖繁殖体系建立

摘要

以越橘品种'科利尔'(Vaccinium vitis-idaea L.'Koralle')茎尖为试材,对影响茎尖离体培养的取材时期、外植体的消毒、培养基和激素配比等因素进行研究,旨在建立茎尖快繁体系.结果表明:休眠期水培促萌的嫩芽污染率较低,最利于茎尖诱导成活.采用正交试验设计筛选出最佳灭菌处理为75%酒精30 s+0.1%升汞4 min+2%次氯酸钠2 min,污染率和成活率分别为5.64%和84.15%;适于茎尖增殖培养的培养基和激素组合为 WPM(改良)+1.0 mg· L-1ZT+0.1 mg· L-1GA3,增殖系数、苗高分别为4.85和2.74 cm.%In order to establish the shoot-tip rapid propagation system for blueberry,we studied the effect of sampling time,ex-plant sterilization,culture medium and hormone combination on Vaccinium vitis-idaea L.'Koralle' shoot-tip.The tender bud,which promoted germination in water during dormant period, had lower pollution rate and higher survival rate.The better sterilization method by orthogonal design was directly surface-sterilized with 75%ethanol for 30 s,0.1%HgCl2for 4 min and 2%NaClO for 2 min,and the pollution rate and survival rate were 5.64%and 84.15%,respectively.The suitable multiplication culture medium and hormone combination for shoot-tip was:improved WPM+1.0 mg· L-1ZT+0.1 mg· L-1 GA3,the multiplication coefficient and seedling height were 4.85 and 2.74 cm,respectively.