用96-Ⅱ喜树无菌苗叶片为外植体,筛选出了96-Ⅱ喜树最佳愈伤组织诱导培养基为WPM+6-BA1.0 mg/L+NAA0.5 mg/L+2,4-D0.1 mg/L,愈伤组织诱导率95％以上；愈伤组织分化培养基为WPM+6-BA0.5 mg/L,分化率达90％；最佳生根培养基为WPM+IBA0.8 mg/L,生根率为94％以上,平均根数为4～8条；移栽成活率达到95％以上.%Adventitious buds were induced using leaf discs from the sterile seedlings of 96-11 Camptotheca acuminata as explants and cultured on WPM, MS and B5 culture media supplemented with various concentrations of hormones 6-BA, NAA and 2,4-D. Results showed that WPM containing 1.0 mg/L 6-BA, 0.5 mg/L NAA and 0.1 mg/L 2,4-D was the optimal medium for callus induction, with a callus induction frequency of 95%. WPM supplemented with 0. 5 mg/L 6-BA was the best medium for the callus differentiation, with a differentiation frequency of 90%. WPM with 0.8 mg/L IBA was the optimal medium for rooting. Four to eight roots appeared and the rooting rate was up to 94%. The survival rate of transplants reached 95%.
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