A psychrotrophic bacterial strain, which can effectively use carbendazim as sole carbon and energy source, was isolated under low temperature conditions from long-term carbendazim administered soil by enrichment screening. Based on the analysis of physiological and biochemical characteristics, 16S rRNA gene sequence homology and phylogenetic tree, the isolate was identified. Its degradation characteristics were tested by HPLC. The bacterial strain was identified as Enterobacter sp. and named as D5. Results show that this strain can utilize carbendazim as sole carbon source for growth under low temperature conditions, and can degrade 100% of carbendazim after 12-day shaking culture at 200 r ·min~-1, 15 degrees C and pH 7. 0. Its degradation rate under the optimal conditions increased by adding exogenous nitrogen source into the medium , but exogenous carbon source had definite inhibition on the degradation of carbendazim by D5.%从长期施用多菌灵农药的土壤中,通过不同温度条件下富集筛选,获得1株耐冷多菌灵高效降解菌株.通过生理生化试验和16 S rRNA序列同源性分析鉴定该菌株；应用高效波相色谱法对纯培养条件下菌株的降解特性进行了分析.结果表明,筛选所获得的菌株与Enterobacter菌属的亲缘关系最近,将其命名为Enterobacter sp.D5；该菌株能在以100 mg·L-1多菌灵为唯一碳源的无机盐培养基中生长；15℃、pH值7.0、200 r·min-1的最适生长条件下避光振荡培养12d,多菌灵的降解率达到100％；在最适培养条件下外加氮源可以提高多菌灵的降解率,外加碳源抑制了多菌灵的降解.
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