不同温度、光照及PEG-6000胁迫对南玉带种子萌发的影响

摘要

以大庆草原干旱生境下采集的南玉带种子为试材,利用控制试验研究了不同光照、温度及聚乙二醇-6000(PEG-6000)胁迫对种子萌发的影响,为构建南玉带种子育苗高质量的技术流程提供依据.结果表明:15～30℃条件下南玉带种子都能够萌发,种子萌发温度范围具有宽适性,其中25℃为种子萌发最适温度；黑暗条件下种子萌发率达92.78％,较光照条件下萌发率高59.45％,其差异显著,说明黑暗条件较光照更适于种子萌发；南玉带种子萌发过程中随PEG-6000质量分数的升高,其萌发率、萌发指数、活力指数均呈下降趋势,但在PEG -6000质量分数为5％、10％处理下种子仍分别有22.78％ 、51.11％的萌发率,说明南玉带种子萌发阶段具有一定的抗旱能力.%Asparagus oligoclonos seeds collected from arid grassland habitats in Daqing were used as test materials. The effects of temperature, light intensity, and water stress induced by polyethylene glycol (PEG-6000) on seed germination of A. oligoclonos were studied in order to provide a scientific basis for the technical process of high quality seedling cultivation from .4. oligoclonos seeds. Results show that the seeds of A, oligoclonos could germinate at 15-30 degrees C, indicating that the seed germination of A. oligoclonos has a wide range of adaptations to temperature. The most suitable temperature for seed germination is 25 degrees C. There is a significant difference in seed germination between dark conditions (92.78%) and light conditions (59.45%), which indicates that dark conditions are more suitable for seed germination. The germination rate, germination index, rigor index of .4. oligoclonos seeds exhibited downward trends with increasing PEG-6000 concentration during germination. However, the germination rates ranged from 22.78% to 51. 11% even under 5% -10% PEG-6000. The germination of A. oligoclonos seeds has a certain resistance to drought during germination.