芽变毛白杨插穗不定根发生的差异蛋白

摘要

A study was conducted to explore the rooting molecular mechanisms of mutant Populusot mentosa carr cuttings.The P. tomentosa carr rooting inhibited and wild-type Populus tomentosa carr rooting induced cuttings as control group , respective-ly.The cutting proteins from Populus tomentosa carr, inhibition of mutant P.tomentosa carr and wild-type P.tomentosa carr rooting were extracted .A total of fourteen differentially expressed proteins were identified by two-dimensional difference gel electrophoresis , among which ten proteins were up-regulated and four proteins were down-regulated .By mass spectrometry and relational databases , above proteins were bark storage protein , chlorophyll a/b binding protein and 60S acidic riboso-mal protein P2.Presumably, these proteins were involved in the mutant P.tomentosa carr cutting adventitious root forma-tion and growth process .%为探索芽变毛白杨（ Populus tomentosa carr）插穗易生根分子机理，分别以芽变毛白杨抑制生根组和野生型毛白杨诱导生根组为对照组，提取芽变毛白杨诱导生根组、抑制生根组和野生型毛白杨诱导生根组插穗蛋白，利用双向差异凝胶电泳技术（2D-DIGE），共检测到了14种差异显著的蛋白质，其中10个为上调表达蛋白，4个为下调表达蛋白。根据质谱鉴定和数据库检索，初步确定上述蛋白为树皮贮藏蛋白、叶绿素a/b结合蛋白和60S酸性核糖体蛋白P2，以及一些未知功能的蛋白，推测这些蛋白质可能参与芽变毛白杨插穗不定根的形成和发育。