RECK及MMP-14在小儿恶性肝脏肿瘤发生和转移中的作用

摘要

ObjectiveTo detect the role of RECK and MMP-14 in occurrence and metastasis of pediatric malignant liver tumor.MethodsImmunohistochemistry was used to detect the expression of RECK and MMP-14 in 32 children with hepatoblastoma tissues,32 children with peritumoral tissues and 30 children with normal liver tissues. And their role in occurrence and metastasis of hepatoblastoma was analyzed.ResultsThe positive rate of RECK expression in hepatoblastoma tissues(37.5%)was significantly lower than the rate in peritumoral tissues(84.3%)(χ2=14.8,P<0.01)and normal liver tissues(86.7%)(P<0.01). However,the positive rate of MMP-14 expression in hepatoblastoma tissues(68.8%)was significantly higher than the rate in peritumoral tissues(21.9%)(P<0.01)and normal liver tissues(23.3%)(P<0.01). The positive rate of RECK expression in children with stageⅠ-Ⅱhepatoblastoma(61.5%)was significantly higher than that in children with stage Ⅲ-Ⅳhepatoblastoma(21.1%)(P<0.05).But the positive rate of MMP-14 expression in children with stageⅠ-Ⅱhepatoblastoma(30.8%)was significantly lower than that in children with stage Ⅲ-Ⅳhepatoblastoma(73.7%)(P<0.05).The rate of RECK expression in 3 years survival group(15.4%)was significantly lower than that in group who lived longer than equarl to 3 years(52.6%)(P<0.05).ConclusionThe expression of RECK and MMP-14 relate to the pathogenesis,progression and prognosis of hepatoblastoma in children.RECK may inhibit the occurrence and metastasis of pediatric malignant liver tumor. But MMP-14 may play a role in facilitation.%目的 探讨富含半胱氨酸的逆转诱导蛋白(RECK)及基质金属蛋白酶-14(MMP-14)在小儿恶性肝脏肿瘤发生和转移中的作用.方法 利用免疫组织化学法检测32例小儿肝母细胞瘤组织(肿瘤组)、32例瘤旁组织(瘤旁组)、30例正常肝脏组织(对照组)中RECK及MMP-14的表达,分析其在肝母细胞瘤的发生和转移中的作用机制.结果 RECK在肿瘤组中的阳性表达率(37.5%)低于瘤旁组(84.3%)(P<0.01)和对照组(86.7%)(P<0.01);MMP-14在肿瘤组中的阳性表达率(68.8%)高于瘤旁组(21.9%)(P<0.01)及对照组(23.3%)(P<0.01);RECK阳性表达率在肝母细胞瘤Ⅰ期和Ⅱ期(61.5%)高于Ⅲ期和Ⅳ期(21.1%)(P<0.05),MMP-14在在肝母细胞瘤Ⅰ期和Ⅱ期的阳性表达率(30.8%)低于Ⅲ期和Ⅳ期(73.7%)(P<0.05);肝母细胞瘤生存期<3年者(15.4%)RECK阳性表达率低于生存期≥3年者(52.6%)(P<0.05),而MMP-14在<3年者的阳性表达率(76.9%)高于≥3年者(36.8%)(P<0.05).结论 RECK及MMP-14的表达与小儿肝母细胞瘤的发生、分期及预后有关,RECK在小儿肝母细胞瘤的发生、发展中可能起抑制作用,而MMP-14则可能起着促进作用.