核小体结合蛋白调控Survivin基因对前列腺癌DU145凋亡的作用

摘要

目的 探讨抑制人核小体结合蛋白1(NSBP1)基因后促进非激素依赖性前列腺癌细胞系DU145凋亡的作用机制.方法 通过慢病毒lentivirus-NSBP1转染非激素依赖性前列腺癌细胞系DU145后,MTT法观察细胞活性变化,流式细胞仪检测细胞的凋亡情况,应用Western-blot技术检测NSBP1、Survivin蛋白的变化情况.结果 抑制NSBP1表达水平后非激素依赖性前列腺癌细胞系DU145细胞活性降低,凋亡增加,同时Survivin蛋白的表达也随之降低.结论 通过慢病毒转染抑制NSBP1的表达可以促进非激素依赖性前列腺癌细胞系DU145凋亡,NSBP1可能是通过调控Survivin基因的变化影响细胞的增值凋亡.%Objective To study the effect and mechanism of nucleosome-binding protein KNSBP1) modulating apoptosis on human androgen-independent prostate cancer cell DU145. Methods After DU145 cells were transfected by lentivirus-NSBP1 ,cell viability was measured with MTT assay,apoptosis was observed with flow cytometry,and the changes of NSBP1 and Survivin expressions were detected with Western-blot method. Results The proliferation of DU145 cells decreased and apop-tosis increased. At the same time,the expression of Survivin protein decreased. Conclusions Inhibited expression of NSBP1 transfected by lentivirus decreased cell proliferation and increased apoptosis of DU145. NSBP1 may affect the cell proliferation and apoptosis by regulating the Survivin protein.