胃癌组织中WISP1和Ki67的表达及其临床病理意义

摘要

目的:探讨胃癌组织中WISP1和Ki67蛋白的表达及其临床病理意义.方法:收集中国医科大学附属第一医院肿瘤外科手术切除的胃癌组织标本87例及其配对非癌(距癌灶边缘＞5 cm取材)胃黏膜组织80例,采用免疫组织化学方法检测WISP1和Ki67蛋白在胃癌组织及其配对正常胃黏膜组织中的表达.结果:胃癌组织中WISP1的阳性表达率为81.61%(71/87),显著高于非癌胃黏膜组织阳性表达率12.50%(10/80).WISP1蛋白表达,在低分化腺癌中的阳性表达较中(P＝0.000)、高(P＝0.032)分化管状腺癌显著增高,弥漫型胃癌显著高于肠型胃癌(P＝0.000),在肿瘤直径＞5 cm的胃癌组织中显著高于肿瘤直径≤5 cm的胃癌组织(P＝0.010),在伴有淋巴结转移的胃原发灶癌组织中较无淋巴结转移组增高(P＝0.025).Ki67蛋白表达与进展期胃癌患者肿瘤浸润深度(P＝0.043)和淋巴结转移(P＝0.027)相关.WISP1阳性表达与Ki67表达呈正相关(rk＝0.240,P＝0.026).结论:WISP1在胃癌组织中表达显著上调,联合检测胃癌组织中WISP1和Ki67蛋白的表达,可更好的预测淋巴结转移情况,为胃癌患者预后评价提供参考.%Objective:To investigate the expression of WISP1 and Ki67 in gastric cancer tissues and its clinico-pathological significance.Methods:87 gastric cancer samples and 80 gastric mucosal tissue (far away from gastric cancer ＞5 cm)were collected from patients who underwent resection of primary gastric cancer at The First Hospital of China Medical University,the expression of WISP1 and Ki67 were examined by immunohistochemistry in gastric cancer tissue and normal gastric mucosa tissues.Results:The positive expression rate of WISP1 in gastric cancer tis-sues was 81.61%(71/87),which was significantly higher than non-cancerous gastric mucosa tissue with positive expression rate of 12.50%(10/80).The expression of WISP1 in poor differentiated adenocarcinoma was significantly higher than well(P＝0.032)and mediate(P＝0.000)differentiate tubular adenocarcinoma.The positive expression of WISP1 was significant higher in patients with diffuse gastric cancer than intestinal type(P＝0.000).The WISP1 expression in patients of gastric cancer with tumor diameter ＞5 cm was significantly higher than the patients with tumor diameter ≤5 cm(P＝0.010).In the tissues of gastric cancer with lymph node metastasis,the positive expres-sion of WISP1 was significantly higher than non-lymph node metastasis(P＝0.025).The expression of Ki67 protein was related to the infiltration depth of tumor in patients with advanced gastric cancer(P＝0.043)and lymph node metastasis(P＝0.027).The relationship between the expression of WISP1 and Ki67 in 87 samples of gastric cancer was positive(rk＝0.240,P＝0.026).Conclusion:WISP1 expression in gastric cancer tissues was significantly high.The joint examination of WISP1 and Ki67 proteins can better predict lymph node metastasis of gastric cancerand can-be used to evaluate prognosisof gastric cancer patients.