沉默环状RNA hsa_circ_0001785表达对乳腺癌MDA-MB-231细胞增殖、迁移及侵袭能力的影响

摘要

目的:探究环状RNA(circRNA)hsa_circ_0001785在乳腺癌组织和细胞中的表达变化及其对乳腺癌细胞增殖、迁移和侵袭能力的影响.方法:qRT-PCR实验检测hsa_circ_0001785在乳腺癌组织和乳腺癌细胞(MDA-MB-231和SK-BP-3)中的相对表达水平;CCK-8和克隆形成实验检测沉默或上调表达hsa_circ_0001785对MDA-MB-231细胞活性和克隆形成能力的影响;划痕愈合实验和Transwell侵袭实验检测沉默或上调表达hsa_circ_0001785对MDA-MB-231细胞迁移及侵袭能力的影响.结果:hsa_circ_0001785在乳腺癌组织中的相对表达水平明显高于癌旁组织,hsa_circ_0001785在MDA-MB-231和SK-BP-3细胞中的相对表达水平明显高于人乳腺上皮细胞MCF10A.在MDA-MB-231细胞沉默hsa_circ_0001785,MDA-MB-231细胞的活性和克隆形成能力明显降低,迁移距离显著减少,侵袭能力也明显下降.而在MDA-MB-231细胞中上调表达hsa_circ_0001785,MDA-MB-231细胞的活性和克隆形成能力显著升高,迁移距离明显升高,侵袭能力也明显升高.结论:hsa_circ_0001785在乳腺癌组织和乳腺癌细胞(MDA-MB-231和SK-BP-3)中的表达水平明显升高;沉默hsa_circ_0001785显著抑制乳腺癌MDA-MB-231细胞的增殖、迁移和侵袭能力,而上调表达hsa_circ_0001785明显促进乳腺癌MDA-MB-231细胞的增殖、迁移和侵袭能力.%Objective:To explore the expression of the circular RNA (circRNA)hsa_circ_0001785 in breast cancer tissues and cell lines,and to study the effect of hsa_circ_0001785 knockdown on the proliferation,migration and invasion of breast cancer cells.Methods:The expression levels of hsa_circ_0001785 in breast cancer tissues and breast cancer cell lines (MDA-MB-231 and SK-BP-3)were measured by quantitative real-time PCR (qRT-PCR).To assess the effect of hsa_circ_0001785 on the viability and proliferation of breast cancer cells,CCK-8 and colony formation assays were performed in MDA-MB-231 cells.Transwell migration and wound scratch assays were carried out to evaluate the effect of hsa_circ_0001785 on the migration and invasion ability of MDA-MB-231 cells.Results:The expression levels of hsa_circ_0001785 were higher in breast cancer tissues and MDA-MB-231 and SK-BP-3 cells than in adjacent normal tissues and human mammary epithelial cells (MCF10A).Knockdown of hsa_circ_0001785 markedly repressed the proliferation,migration and invasion of MDA-MB-231 cells.Besides,upreg-ulation of hsa_circ_0001785 promoted the proliferation,migration and invasion of MDA-MB-231 cells.Conclu-sion:Knockdown of hsa_circ_0001785 remarkably represses the proliferation,migration and invasion of MDA-MB-231 cells.