FASCIN-1和MMP-1在喉鳞癌中的表达及其临床意义

摘要

目的:探讨Fascin-1和MMP-1在喉鳞状细胞癌(LSCC)和癌旁组织中的表达及意义.方法:免疫组化SP法检测50例喉鳞状细胞癌及其癌旁0.5cm、1.0cm、2.0cm组织中Fascin-1和MMP-1的表达情况,并分析两者的关系.结果:Fascin-1在喉鳞癌中的表达及癌旁0.5cm、1.0cm及2.0cm的黏膜中表达率依次降低,分别为80.0%、27.5%、22.5%、4.0%,除了癌旁0.5组与癌旁1.0组,癌旁1.0组与癌旁2.0组差别无统计学意义之外(P＞0.008),其余各组间差别均有统计学意义(P＜0.008).MMP-1在喉癌癌旁0.5cm、1.0cm及2.0cm的表达率依次降低,分别为85.0%、35.0%、17.5%、5.0%,除了癌旁0.5组与癌旁1.0组,癌旁1.0与癌旁2.0差别无统计学意义之外(P＞0.008),其余各组间差别均有统计学意义(P＜0.008).喉鳞癌中Fascin-1蛋白的表达与癌的外科切缘,临床分期,肿瘤分化程度及淋巴结转移相关,与性别和年龄无关.MMP-1蛋白表达同样与癌的外科切缘,临床分期,肿瘤的分化程度及淋巴结转移相关,与性别和年龄无关.二者的表达呈正相关(P＜0.001).结论:Fascin-1和MMP-1在喉鳞癌侵袭和转移中发挥重要作用,二者联合检测可能成为预测喉鳞癌预后的生物学指标,并为以Fascin-1和MMP-1为靶点的癌症治疗提供了思路.%Objective : To study the expression of Fascin - 1 and MMP - 1 ( matrix metalloproteinase - 1 ) in laryngeal squamous cell carcinoma ( LSCC ) and adjacent tissues and their clinical significance. Methods : Using citric SP - immunochemical technique , the expression of Fascin - 1 and MMP - 1 were studied in different regions of larynx squamous cell carcinoma including 40 cases laryngeal carcinoma and adjacent tissues which was 0. 5 , 1. 0,2. 0 cm away from cancer tissue. Results : The expression of Fascin - 1 reduced in sequence cancer tissue , at 0. 5 cm , 1 . 0 cm , 2. 0cm adjacent to carcinoma , which were 80. 0％ ,27 . 5 ％ ,22. 5％ ,4. 0％ , there was no significant difference in rate of addition ( P ＞0. 008 ) in group of 1.0cm and 2. 0 cm and the group of 0. 5cm and 1. 0cm,the other groups differences were statistically significant ( P ＜ 0. 008 ). The expression rates of MMP - 1 in cancer tissue , at 0. 5cm , 1.0cm , 2. 0cm adjacent tissaes were 85 . 0％ , 35. 0％ ,17. 5 ％ ,5. 0％ , there was no significant difference ( P ＞ 0. 008 ) group between 1.0cm and 2. 0 cm and the group of 0. 5cm and 1.0cm,in other groups differences were statistically significant ( P ＜0. 008 ). The expression of Fascin - 1 protein in Laryngeal squamous cell carcinoma had a relationship with clinical stage of cancer, tumor location, differentiation and lymph node metastasis, but not associated with age and gender. The expression of MMP - , have a relationship with clinical stage of cancer, tumor location, tumor differentiation and lymph node metastasis , but not associated with age and gender. the expression of Fascin - 1 and MMP - 1 were positively correlated( P ＜ 0. 001 ). Conclusion : Fascin - 1 and MMP - 1 maybe directly associated with the development of LSCC,which may play an important role in the process of invasion and metastasis of LSCC, combined detection of Fascin - 1 and MMP - 1 may be an important biological factor affecting the prognosis judgement of LSCC.