hTERT 反义寡核苷酸对脊索瘤细胞周期及增殖的影响

摘要

Objective : To investigate the effect of inhibiting telomerase activity by hTERT antisense oligodenxynucleotide on proliferation of chordoma cell line CM319. Methods : Human chordoma cell line CM319 was transfected with hTERT antisense oligodenxynucleotide ( ASODN ) and sense oligodenxynucleotide ( SODN ), mediated by lipofectamine. MTT was used to examine cell activity. The cell cycle and the rate of apoptosis were determined by flow cytometry 72 hours after treatment. Results: The telomerase activity and the proliferation were markedly depressed in CM319 cell which was transfected with hTERT ASODN. The cell cycle was arrested in S phase, and early apoptotic peak was showed by flow cytometry. The rate of apoptosis was 16. 01％ while the control group was 2. 3％ . Conlusion : ASODN targeting hTERT can inhihit the proliferation of chordoma cell line CM319 and induce apoptosis.%目的:探讨hTERT反义寡核苷酸(ASODN)抑制CM-319细胞端粒酶活性对脊索瘤细胞的细胞周期及增殖的影响.方法:用脂质体介导hTERT正义寡核苷酸(SODN)和反义寡核苷酸(ASODN)转染CM-319 脊索瘤细胞株72小时后,MTT 法检测细胞活性,流式细胞仪检测细胞周期及细胞凋亡率变化.结果:hTERT 的ASODN转染细胞的端粒酶活性及细胞生长都明显受到抑制.流式细胞仪检测显示:ASODN组细胞出现早期凋亡峰,细胞阻滞于S 期,而hTERT 的SODN则无此作用.ASODN组细胞凋亡率为16.01%,而hTERT 的SODN组为2.3%.结论:端粒酶hTERT为靶点的反义寡核苷酸可明显抑制脊索瘤细胞的增殖,且具有促凋亡作用.