恶性胸腔/腹腔积液RRM1mRNA表达水平和吉西他滨体外药敏的关系

摘要

Objective: To investigate the association of mRNA expressions of RRM1 ( ribonucleotide reductase M1 ) with chemosensitivity to gemcitabine in malignant pleural and peritoneal effusions. Methods : Malignant pleural and peritoneal effusions were collected from 24 patients diagnosed with stageⅣ malignant tumor, prospectively. The tumor cells were isolated and the sensitivity of tumor cells to gemcitabine was detected by CCK - 8 assays. Real time quantitative PCR was used to determine the mRNA expressions of RRM1. Results: The expression level of RRMI was no relation with clinical feature, but negatively correlated with sensitivity to gemcitabine ( r = - 0. 497 ,P = 0. 013 );Patients who were not treated by chemotherapy seemed more sensitive to gemcitahine than those who were treated with chemotherapy previously ( 42. 94％ vs67. 88％ ,P =0. 006 ). Conclusion : RRM1 mRNA expression level was correlated with ex vivo chemosensitivity of tumor cells to gemcitabine in malignant pleural and peritoneal effusions.%目的:分析恶性胸腔/腹腔积液中肿瘤细胞核糖核苷酸还原酶M1亚基(ribonucleotide reductase M1,RRM1)基因的表达水平与吉西他滨体外药物敏感性的关系.方法:收集24例经病理确诊的Ⅳ期恶性肿瘤患者的恶性胸腔/腹腔积液,分离原代肿瘤细胞,采用CCK8(cell counting kit8)法体外药物敏感试验检测原代肿瘤细胞对吉西他滨药物敏感性;实时荧光定量PCR检测原代肿瘤细胞RRM1 mRNA的相对表达水平.结果:在恶性胸腔/腹腔积液中,肿瘤细胞的RRM1 mRNA的相对表达水平与恶性肿瘤患者的临床特征无明显相关性,但是与吉西他滨药物敏感性呈负相关(r=-0.497,P=0.013);既往曾使用化疗药物的恶性肿瘤患者恶性胸腔/腹腔积液原代肿瘤细胞对吉西他滨敏感性低于未化疗患者(42.94%vs67.88%,P=0.006).结论:恶性胸腔/腹腔积液中肿瘤细胞RRM1 mRNA的相对表达水平与吉西他滨体外药物敏感性相关.