Objective :To study the effect of Ad - PTEN gene in SGC - 790ltumor cells. To study the inhibition and potential molecular mechanisms of adenovirus - mediated expression of Ad - PTEN gene in SGC - 7901 gastric cancer cells. Methods : PTEN gene was transfected into human gastric: cancer cell line SGC - 7901 with a replication - incompetent adenovirus vector. Using RT - PCR to detect the mRNA expression of PTEN. Using light microscope and fluorescence microscope observe the appearances of SGC - 7901 cells infected by Ad - PTEN . Using MTT essay and flow cytometry to test the cell growth and apoptotic effect. Using RT - PCR to analyze apoptosis related gene expression such as Bax, Bcl -2, p53 , Survivin. Results : Ad - PTEN gene infect SGC - 7901 gastric cancer cells. RT - PCR revealed that the transcription of PTEN gene and its expression can inhibit SGC - 7901 gastric cancer cell growth. Apoptosis may be related Bax/Bcl - 2 ratio increased, Survivin decreased, p53 elevated. Conclusion : Recombinant adenovirus expressing Ad - PTEN can inhibit the growth of SGC -7901 gastric cancer cells and induce its apoptosis.%目的:探讨腺病毒介导的PTEN基因表达体外对SGC-7901胃癌细胞生长抑制作用及其分子机制.方法:将携有PTEN基因的复制缺陷型腺病毒载体(Ad-PTEN)感染SGC-7901胃癌细胞,用RT-PCR法检测Ad-PTEN在细胞中的表达,光学显微镜及荧光显微镜下观察Ad-PTEN感染细胞前后形态的变化,MTT法检测Ad-PTEN对SGC-7901胃癌细胞生长的抑制作用,用流式细胞术(FCM)检测SGC-7901胃癌细胞凋亡率.RT-PCR分析Bax、Bcl-2、p53、Survivin细胞凋亡相关基因的表达.结果:Ad-PTEN基因组感染SGC-7901胃癌细胞后,RT-PCR结果显示PTEN目的基因能在SGC-7901胃癌细胞中转录,其表达可明显抑制该胃癌细胞的生长,并诱导细胞凋亡.其凋亡机制可能与Bax/Bcl-2比值、p53基因表达上调、Survivin下调有关.结论:重组腺病毒Ad-PTEN具有抑制SGC-7901胃癌细胞生长和诱导细胞凋亡的作用.
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