端粒酶活性的原位检测在大肠癌早期诊断中的意义

摘要

目的:研究端粒酶活性的原位检测在大肠癌早期诊断中的意义.方法:运用原位杂交技术检测30例大肠癌组织、癌旁组织及其正常黏膜中端粒酶mRNA的表达情况.结果:在30例大肠癌中,端粒酶mRNA的阳性表达率为90.0%,明显高于癌旁组织、正常黏膜组织(P＜0.005).端粒酶活性的表达强度与肿瘤的浸润深度、临床分期以及淋巴结转移显著相关(P＜0.05),与性别、年龄以及肿瘤的分化程度无相关性.结论:端粒酶的激活在大肠癌的发生、发展中起到一定的作用,可以将端粒酶作为一种新的肿瘤标记,通过对细胞端粒酶活性的原位检测来协助大肠癌的早期诊断.%Objective: To study the significance of telomerase activity in the early diagnosis of colorectal carcinoma.Methods: Telomerase activity was examined by using In Situ hybridization mRNA of telomerase in cancer tissues,adjacent cancer tissues, normal tissues from patients with colorectal carcinoma.Results: Twenty seven of 30 ( 90.0％ ) exhibited telomerase activity in cancer tissues compared with 3/30 ( 10.0％ ) in adjacent cancer tissues,0/30 in normal tissues.There was significant difference between them( P ＜ 0.005 ).Telomerase activity level in colorectal carcinoma was significantly associated with the invasion, clinicopathologic stage and lymph node metastasis ( P＜ 0.05 ).Conclusion: The expression of telomerase activity plays some role in development of colorectal carcinoma.Telomerase can be a new tumor marker for colorectal carcinoma.There is significance to detect telomerase activity in situ for early diagnosis of colorectal carcinoma.