Objective: To study the Correlation between expression of Cyclin E in breast cancer and invasion of primary breast cancer cells. Methods: 1cm3 of the breast cancer tissue was taken got from operation, prepareing breast cancer cells suspension of origin generation, and detecting the expression level of Cyclin E by immunofluorescent technique. These cancer cells of origin generation were detected for invasion capability. Results: The invasion test presented that the average numher of cells of origin generation with overexpression of Cyclin E infiltrating Transwell PC micropore film was 76. 32 ± 11. 40 per high power field, the average number of cells of origin generation with negative expressivion of Cyclin E was 21. 56 ± 13. 22 per high power field P ＜ 0. 05. Conclusion : The expression of Cyclin E is related with the invasion of origin generation cells in breast cancer, the invasion of overexpression of Cyclin E is more powerful than negative expression of Cyclin E in breast cancer.%目的:从细胞学角度探讨Cycli E表达水平与原代乳腺癌细胞侵袭之间的关系.方法:手术中无菌条件下切取乳腺癌组织,制备原代乳腺癌细胞悬液,运用Transwell小室进行原代乳腺癌细胞侵袭力实验;获取具有不同侵袭力的原代乳腺癌细胞,再用免疫荧光技术检测不同侵袭能力的原代乳腺癌细胞cyclin E的表达水平,研究两者相关性.结果:具有不同侵袭力的乳腺癌细胞表达不同强度的Cyclin E蛋白,分别计数5个高倍视野,其均数分别为81.11 ±17.25及 31.06±4.43,P< 0.05.侵袭性试验结果显示:Cyclin E阳性表达的原代乳腺癌细胞经24 小时培养以后浸润到Transwell聚碳酸盐微孔滤膜下 的细胞数的均值为:76.32±11.40/高倍视野;而Cyclin E阴性表达的原代乳腺癌细胞的均值为21.56±13.22/高倍视野;P< 0.05.结论:Cyclin E的表达差异与原代乳腺癌细胞的侵袭性有关,Cyclin E高表达者其侵袭性显著强于Cyclin E低表达者.
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