改良MTT药敏分析在成骨肉瘤个体化治疗中的意义

摘要

目的:为提高骨肉瘤患者的治疗有效率,通过改良MTT药敏分析,研究成骨肉瘤药物敏感性,探讨成骨肉瘤个体化治疗的可行性.方法:通过收集新鲜骨肉瘤组织收集培养骨肉瘤细胞,以MTT比色法观察6 种常用化疗药对骨肉瘤细胞增殖的影响.结果:骨肉瘤细胞对MTX,紫杉醇和5-FU 敏感性强,生长抑制率达到50.06%,48.35%和47.13%.结论:通过对骨肉瘤细胞进行MTT药敏分析研究,选择性地对患者开展个体化化疗,对提高骨肉瘤治愈率有重要的意义.%Objective : To study osteosarcoma drug sensitivity and improve the effect of osteosarcoma treatnent.Methods :Osteosarcoma cells were prepared from fresh osteosarcoma tissues.Observing the influence of six kinds of chemotherapeutic drugs to the cell proliferation of osteosarcoma cell by MTT colorimetry.Results : Osteosarcoma cells were more sensitive to MTX, Paclitaxe and 5 - FU.The result of MTT showed the inhibitoy rates were 50.06％ ,48.35％ and 47.13％ respectively.Conclusion: The chemotherapeutics drug sensitivity test of osteosarcoma cells plays an important role to improve curative effect.