肝细胞生长因子cDNA真核表达载体的构建

摘要

目的:构建肝细胞生长因子cDNA真核表达载体.方法:提取大鼠胎肝总RNA,利用RT-PCR技术扩增出肝细胞生长因子cDNA,连接于载体pMD18-T质粒上,构建重组载体,经双酶切和DNA测序分析检测重组载体构建结果.结果:成功构建肝细胞生长因子cDNA真核表达载体.结论:为进一步研究HGF与肝癌的关系提供了实验基础.%Objective :To construct expresaion vector of HCF cDNA.Methods : We extracted the fetal liver cotal RNA, used RT - PCR to amplify HGF cDNA, and then cloned them in pMD18 - T vector to construct recombinant vectors.The result af recombinant vector was identified usinS PCR and DNA sequencing.Results: HCF cDNA expression vector was constructed successfully.Conchsion : Constructing HGF cDNA expression vector may provide an experimental basis for the further study of the relationship between HGF and hepatic carcinoma.