循环肿瘤细胞自我种植对裸鼠骨肉瘤生长的促进作用

摘要

目的:通过建立骨肉瘤裸鼠荷瘤模型,观察骨肉瘤中是否存在循环肿瘤细胞自我种植现象,并探讨其在骨肉瘤进展中的作用.方法:建立骨肉瘤裸鼠原位荷瘤模型后,处理组尾静脉注射红色荧光蛋白(RFP)标记循环肿瘤细胞,对照组注射PBS,2周后将处理组原位瘤体冰冻切片荧光显微镜下观察有无自我种植,并根据肿瘤生长指标(大小、重量、生长曲线)将处理组与对照组进行比较分析.结果:处理组瘤体冰冻切片后荧光显微镜下观察发现,原位瘤组织中有红色荧光散在分布,即存在循环肿瘤细胞自我种植现象.肿瘤生长各指标比较发现,处理组瘤体在大小、重量、生长速率上均显著高于对照组(P＜0.05),具有统计学意义.结论:骨肉瘤中存在循环肿瘤细胞自我种植现象,并且能够促进原位灶的生长.%Objective: To observe whether self - seeding of circulating tumor cells is existing in osteosarcoma, and to explore its role in the progression of osteosarcoma through the establishment of nude mice model transplanted with human osteosarcoma cell line. Methods: We established an orthotopic model of human osteosarcoma by transplanting tumor cells into tibial plateau of nude mice. The treatment group received injection of circulating tumor cells labeled by red fluorescent protein( RFP) in tail vein, while the control group getting equal volume of PBS. After 2 weeks, tumor tissues of the treatment group were collected to make frozen sections in order to observe whether the self — seeding existing under the fluorescence microscope. And some indicators of tumor growth ( size, weight, growth curve) were compared between the treatment group and control group. Results: We observed that red fluorescence scattered in the frozen sections of situ tumor tissues under the fluorescence microscopy, implying the existence of circulating tumor cell (CTC) self - seeding. The comparison between the two groups indicated that the indicators of tumor growth of the treatment group (tumor size, weight and growth rate) were all significantly higher than that of the control group (P<0.05) ,with significant statistical difference. Conclusion: CTC self-seeding exists in osteosarcoma, and it can promote the growth of primary tumor.