肺癌患者EGFR基因启动子甲基化状态研究

摘要

Objective: To detect the EGFR gene methylation state and discuss the relationship between the clinical characteristics,EGFR mutation,and the EGFR methylation of lung cancer,providing a theory basis for target therapy for lung cancer. Methods: The methylated DNA was amplified by PCR and then sequenced directly. We investigated the pathological characteristics of these patients that had been stratified according to methylation mmu. Edu. Comsta-tus. Results:The EGFR gene promoter methylation was observed in 23 of 76 NSCLC samples examined. The methylation rate was 30. 3% . The methylation state of patients had no significant correlation with gender, age, smoking history, lymph node metastasis, infiltrating degree and TNM stage and was related to the pathological type. In the second part of this research,we could see that the EGFR methylation was related to the mutation type. Conclusion:The EGFR gene promoter methylation was observed in lung cancer. There is a closed relationship between EGFR methylation and mutation type.%目的:探讨肺癌组织中EGFR基因启动子的甲基化水平及甲基化状态与EGFR外显子突变的关系.方法:应用聚合酶链技术、基因克隆测序技术和荧光偏振技术,检测肺癌患者病理组织中EGFR基因启动子的甲基化水平.结果:76例肺癌组织中EGFR基因启动子甲基化阳性者23例,检出率为30.3％,甲基化状态与肺癌患者的性别、年龄、吸烟史、淋巴结转移、浸润程度和TNM分期均无明显相关性,与患者的病理类型密切相关,吸烟对甲基化的相对危险度(OR)为1.88,40例NSCLC患者中,29例未检测到敏感突变的患者中有10例甲基化为阳性,甲基化率为34.5％,而11例TKI敏感突变的患者中均未检测到甲基化.结论:肺癌患者EGFR基因存在启动子甲基化,EGFR外显子的突变状态与启动子甲基化有密切关系.