P-gp、GST-π和TopoⅡ在涎腺黏液表皮样癌中的表达及意义

摘要

目的:探讨涎腙黏液表皮样癌多药耐药基因P-gp、GST -π和TopoⅡ的表达情况及意义.方法:应用免疫组织化学技术(SP法)检测37例涎腺黏液表皮样癌及12例正常涎腺组织中P-gp、GST -π和TopoⅡ的表达.结果:P-gp、GST -π和TopoⅡ在涎腺黏液表皮样癌及正常涎腺组织中均有不同程度表达.涎腺黏液表皮样癌组织中P-gp、GST -π和TopoⅡ表达阳性率分别为91.9％ (34/37) 86.5％ (32/37)和21.6％ (8/37).TopoⅡ的表达与肿瘤的分化程度呈负相关.结论:P-gp、GST -π和TopoⅡ在涎腺黏液表皮样癌的多药耐药机制具有重要作用.检测P-gp、GST -π和TopoiⅡ可为临床拟定化疗方案提供依据,也可作为判断涎腺黏液表皮样癌预后的参考.%Objective:To investigate the clinical significance of P - glycoprotein, GST - π and TopoⅡ expression in mucoepidermoid carcinoma of the salivary glands. Methods: The expression of P - glycoprotein、GST - π and TopoII in 37 cases of epidermoid carcinoma and 12 cases of normal salivary glands was detected immuohistochemically by SP method. Results: P - glycoprotein,GST - π and TopoⅡ expression was found in both mucoepidermoid carcinoma and normal salivary glands. The positive rates of P - glycoprotein, GST - π and TopoII in mucoepidermoid carcinoma of salivary glands were 91.9% ( 34/37), 86.5% (32/37 ) , 21.6% ( 8/37 ). There was a negative correlation between the expression of TopoⅡ and degree of differentiation. Conclusion; P - glycoprotein, GST - π and TopoⅡ play an important role in the multidrug resistance in epidermoid carcinoma of the salivary glands. P - glycoprotein, GST - π and TopoⅡ detection may be helpful to chemotherapy and should also be considered as a significant indicator of prognosis in epidermoid carcinoma of the salivary glands.