bcl-2基因表达和其它肿瘤标志物在乳腺癌诊断中的应用

摘要

Objective:To discuss clinical application of bcl -2 gene expression in the diagnosis and therapy of breast cancer. Methods: Clinical samples of 20 health women, 81 patients with benign breast disease and 56 patients with malign breast disease were detected by FQ - PCR. CA153 ,CA125 and CEA were detected by chemical luminous method, and the results were analyzed. Results: The sensitivity of bcl -2 to diagnosis breast cancer was higher than those of CA153,CA125 and CEA. Four index were declining after operation and chemotherapy. Conclusion : Gene expression of bcl - 2 can give objective evidence to the diagnosis, therapy and prognosis of breast cancer.%目的:探讨bcl-2基因表达在乳腺癌诊断和治疗监测中的应用.方法:采用荧光定量PCR(FQPCR)技术检测20例健康女性体检者、30例良性乳腺肿瘤和81例乳腺癌外周血中bcl -2基因表达,化学发光法检测其CA153、CA125和CEA,并进行对比分析.结果:bcl-2对乳腺癌诊断灵敏度显著高于CA153、CA125和CEAo四种指标在手术和化疗后均显著下降.结论:bcl -2基因表达水平可有效监测乳腺癌的诊断、治疗和预后.