目的:明确miR-125b在子宫内膜癌中对HER2基因3'UTR的靶向调控作用.方法:分别构建野生型和突变型HER2基因 3'UTR荧光素酶载体;软件预测HER2基因 3'UTR靶向miRNAs;共转染miR-125b和荧光素酶载体于HEC-1B细胞中,双荧光素酶检测系统测定荧光素酶活性.结果:通过软件预测在HER2 3'UTR第37bp位置有一个miR-125b结合位点;与转染对照miR组和突变型载体组相比,miR-125b前体能明显降低野生型载体的荧光素酶活性.结论:miR-125b能够靶向负性调控HER2基因 3'UTR活性.%Objective: To identify the regulation of miR - 125b to HER2 gene 3 UTR in endometrial cancer. Methods: The wild type and mutation type of HER2 gene 3 UTR luciferase vectors were constructed. Software was applied to predict the HER2 3 UTR targeting miRNAs. The dual luciferase assay system was used to detect the reporter activity followed by co - transfecting the luciferase vector constructed and pre - miRNA into HEC - IB cells. Results: MiRNA targeting prediction showed that the 37th bp in 3 UTR contained a miR - 125b binding site. The luciferase assay revealed that pre - miR - 125b could significantly decrease the luciferase activity of wild type HER2 3 UTR vector, compared with the control and mutation vector transfection groups, P < 0.05. Conclusion: MiR - 125b could negatively target and regulate the activity of HER2 gene 3 UTR.
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